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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become noticed in several tumors, which leads to chromosome instability, and so may contribute to tumorigenesis. This protein was identified to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a vital part in selling cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are actually explained. Two added splice variants have been reported but detailed nucleotide sequence information and facts is not really still obtainable.
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Blocking peptides are peptides that bind exclusively into the target antibody and block antibody binding. These peptide ordinarily includes the epitope identified from the antibody.
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This guideline outlines additional achievable optimization of cellular and immunoassay parameters to guarantee the very best success are...
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This information outlines more feasible optimization of mobile and immunoassay parameters to guarantee the best possible effects are...
Antibodies certain to the blocking peptide no longer bind to your epitope within the target protein. By comparing the staining within the blocked antibody vs . the antibody alone, one can see which staining is unique.
Goal Facts Cyclin E belongs towards the remarkably conserved cyclin family members, whose customers are characterised by a dramatic periodicity in protein abundance throughout the mobile cycle. Cyclins functionality as regulators of CDK kinases. Various cyclins exhibit unique expression and degradation designs which add to the temporal coordination of each mitotic occasion. Cyclin E types a fancy with and features like a regulatory subunit of CDK2, whose action get more info is required for cell cycle G1/S changeover. This protein accumulates at the G1-S section boundary and is degraded as cells development by S period.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both of those antibodies...
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